CD3 anticorps

N° du produit ABIN6961433

Cet anticorps Souris Monoclonal détecte spécifiquement CD3 dans FACS, IP, IHC (fro), Func, IF et WB. Il présente une réactivité envers Humain.

Aperçu rapide pour CD3 anticorps (ABIN6961433)

Antigène: CD3

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunofluorescence (IF), Western Blotting (WB)

Clone: UCHT1

Détail du produit anti-CD3 anticorps

Réactivité croisée: Chimpanzé

Purification: This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.

niveau d'endotoxine: Low endotoxin: < 0.01 EU/ug

Isotype: IgG1 kappa

Information d'application

Indications d'application: This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.

Commentaires:

2 mg/mL

Restrictions: For Research Use only

Stockage

Buffer: 10 mM NaH2PO4, 150 mM NaCl, pH 7.2

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: 2-8°C

Date de péremption: 12 months

Détails sur CD3

Antigène: CD3

Autre désignation: CD3

Sujet: The UCHT1 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor - CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell ac- tivation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The UCHT1 antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of UCHT1 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a CD3e- antibody complex provides insight as to the action of commonly used agonist antibodies, as well as specific epitope-binding data for the human CD3 antibodies UCHT1 and OKT3 (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).UCHT1 antibody reacts with both surface-expressed and intracellular CD3e protein, in contrast to an alternative human CD3 clone, HIT3a, which will stain only the extracellular (membrane-expressed) CD3e protein. Also, the UCHT1 antibody is reported to be cross-reactive with chimpanzee and has been used for phenotypic analysis of expression by flow cytometry - however the antibody is reported to be unsuitable for induc- tion of T cell activation in this species (Bibollet-Ruche et al. 2009. J. Virol. 82: 10271-10278).

ID gène: 915, 916, 917

UniProt: P20963, A8MVP6, P07766, P09693

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway